Summaries :
- Microbes like bacteria, algae, and fungi are essential for biomass fermentation to occur. It’s crucial to select microbe carefully.
- The fermentation process lasted for 72 hours. The findings indicated that the ideal concentration of waste cooking oil with an organic nitrogen source of peptone at 70 g/L was 80 g/L, while the optimal inorganic nitrogen source from urea at 40 g/L resulted in a protein content of 5.06%.
- Mycelia can be redesigned to utilize different by-products and waste materials from agriculture and forestry.
Among all alternative protein technologies, biomass fermentation presents one of the greatest chances to produce protein on a large scale with minimal or no processing needed to create a final product. One such example is Microorganism or Microbiobe that assists in producing biomass products that also involves with some feedstocks
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Microbial Biomass
Microbial biomass refers to the overall count of microorganisms, which includes the total of bacteria, fungi, and protozoa. The microbial biomass serves as a sign of overall disease suppression. The greater the number of microorganisms, the higher the competition with pathogens for resources and habitat.
The microbial biomass can be enhanced by incorporating beneficial organic materials like compost, solid manure, green manures, or growing grains (including straw). Additional examples of measures include reduced tillage, continuous soil coverage, temporary grassland, or decreased ploughing of permanent grasslands.
Microbes like bacteria, algae, and fungi are essential for biomass fermentation to occur. It’s crucial to select microbe carefully.
From well-known strains to new extremophiles, companies can utilize the diversity of microorganisms as a valuable resource. Microbes can differ in their effectiveness, production, nutritional composition, and functional outcomes. This also indicates that they will have distinct production needs.
Businesses might consider utilizing a well-documented microorganism, because these often already possess GRAS (generally regarded as safe) classification and have been successfully employed in various food applications.
In recent years, Yellowstone’s extremophiles have become a focus of scientific study and have produced intriguing strains suitable for biomass fermentation.
To identify a new candidate strain capable of yielding high-quality protein and/or valuable metabolites, companies can explore microbial libraries and databases. Alternatively, others might choose to investigate natural regions for commercially-viable native species through a methodical technique referred to as bioprospecting.
To discover a new candidate strain that generates high-quality protein and/or valuable metabolites, companies may explore microbial libraries and databases. On the other hand, some individuals might desire to investigate natural environments to find commercially-valuable native species using a methodical strategy called bioprospecting.
Carbon within Microbial/Bacterial/Fungal Matter
Microbial biomass serves as a significant measure of soil vitality. Carbon in microbial biomass (mg C kg -1) is derived from total microbial biomass (mg PLFA per kg).
It is also used as a metric frequently utilized in various measurements and research studies. The report includes carbon from microbial, bacterial, and fungal biomass to allow for comparisons.
Lignocellulosic biomass, commonly referred to as lignocellulose, is plant-derived biomass mainly made up of lignin, cellulose, and hemicellulose. These three elements constitute the cell walls of plants and serve as essential building blocks for lignocellulosic biomass. It is an important renewable resource and a possible feedstock for multiple uses, such as biofuel generation.
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Rhizopus Oryzae on Biomass
Rhizopus oryzae generates the lipase enzyme that hydrolyzes triglycerides into glycerol and fatty acids. This study aimed to identify the ideal concentration of waste cooking oil substrate and the best concentration of inorganic urea nitrogen source during fermentation for biomass protein production.
The starting waste cooking oil substrate concentration varied between 60 g/L and 100 g/L, whereas the urea nitrogen source concentration ranged from 30 g/L to 70 g/L.
The fermentation process lasted for 72 hours. The findings indicated that the ideal concentration of waste cooking oil with an organic nitrogen source of peptone at 70 g/L was 80 g/L, while the optimal inorganic nitrogen source from urea at 40 g/L resulted in a protein content of 5.06%.
Raw Materials
Additionally, advancements have been achieved in synthetic biology, in which microbes can be altered to utilize a diverse range of substrates. Progress has also been achieved in leveraging side streams from various industries to establish circular processes, including apple pomace and potato wastewater.
Just as we cannot depend solely on filamentous fungi for all plant-derived meat alternatives, we should not concentrate exclusively on glucose derived from corn and wheat as the singular feedstock in the biomass fermentation process.
The challenge is in locating source materials that can be processed into effective feedstocks without needing complicated treatment. However, as technology advances, it may become indifferent to different carbon sources, including lignin, cellulosic, and glucose.
Mycelia can be redesigned to utilize different by-products and waste materials from agriculture and forestry.
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Editor: Tri Indah Lestari
References :
[1] Fermentasi Minyak Jelantah Menggunakan Rhizopus Oryzae Untuk Menghasilkan Protein Biomassa
[2] Biomass Fermentation: The Most Flexible Alt Protein Technology?
